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FLOW CYTOMETRY CORE FACILITY


FACS Calibur

FACS Vantage SE

LOCATION:

The Flow Cytometry laboratory is a core facility located in the IDM Building, Room S1.11,  Division of Immunology, Faculty of Health Sciences, University of Cape Town. It runs under the auspices of Professor Frank Brombacher.

CONTACT:

Ronnie Dreyer
Tel:  021 406 6076
Cell: 083 461 8030
email: ronnie.dreyer@uct.ac.za

EQUIPMENT:

FACS Calibur:

  • Acquisition and analysis of samples.
  • Measures 6 parameters: FSC (size), SSC (granularity), FITC, PE, PERCP and APC.
  • Equipped with a 488nm argon laser and a 635nm diode laser.
  • Uses a Power Macintosh G4 system for data analysis.

FACS Vantage SE:

  • CELL SORTING of cells/particles (0.4 to 200 microns).
  • Measures 7 parameters: FSC, SSC and FITC, PE, PERCP, APC, and APC Cy7.
  • ULTRA VIOLET Cell sorting or FACS analysis as well as OTHER COLOURS available on request.
  • Equipped with a water-cooled laser (Coherent Innova 300C) and a Spectrum Physics laser.
  • Uses a Power Macintosh G4 system for data analysis.
  • The following sorts are available:
    • LOW/HIGH SPEED SORT
    • STERILE SORT
    • SINGLE CELL/CLONE SORT (6 - 96 well plates & petri dishes)

FACS Aria Cell Sorter:

Information to follow

 

   More details on cell sorting here

RATES:  FACS CALIBUR

ACQUISITION:      Academic - By user: R286/hr / By operator: R386/hr
                           Commercial  - By user: R286/hr / By operator: R484/hr

ANALYSIS:            Academic - By user: R286/hr   / By operator: R386/hr
                           Commercial - By user: R286/hr / By operator: R386/hr

 

RATES:  CELL SORTING

ACQUISITION:     Academic - R386/hr / Commercial - R539/hr

ANALYSIS:           Academic - R386/hr / Commercial - R539/hr

 

 

HISTOLOGY CORE FACILITY

Histology Histology Histology

 

LOCATION:

The Histology Core Facility is located in the Old Main Building, Department of Surgery, J Floor, Groote Schuur Hospital, Observatory in Cape Town. The facility comes under the auspices of the Division of Immunology in the Department of Pathology, and the IDM, Faculty of Health Sciences, University of Cape Town.

CONTACT:

Lizette Fick
Tel: 021 406 6227
Email: lizette.fick@uct.ac.za

SERVICES OFFERED:

  • Trimming of Formalin Fixed Tissue
  • Tissue Processing
  • Embedding of Processed Tissue
  • Cutting of Sections
  • Histochemical Staining of Sections
  • Immunohistochemical Staining of Sections
  • Cutting and Staining of Cryosections

 

 

GENOME PLATFORM SERVICES

Three GENOME PLATFORM SERVICES are available:

DNA sequencing using the ABI 3100 Genetic Analyzer, which is capable of sequencing 16 samples per hour by capillary electrophoresis.

DNA genotyping using the ABI 3100 Genetic Analyzer which is capable of handling 2 X 96 well plates in a single overnight run.

The WAVE mutation detection screening system which utilises DHPLC (denaturing high performance liquid chromatography).

For more information »

 

 

 
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